Synthesis of ω‐Muricholic Acid by One‐Pot Enzymatic Mitsunobu Inversion using Hydroxysteroid Dehydrogenases

Enzymatic estimation of steroids in subpicomole quantities by hydroxysteroid dehydrogenases and nicotinamide nucleotide cycling.

Extremely sensitive methods are described for the measurement of 3 alpha- and 3 beta-hydroxysteroids, as well as 3-ketosteroids, based on their reaction with highly purified bacterial hydroxysteroid dehydrogenases and the amplification of the accompanying changes in nicotinamide nucleotides by enzymatic cycling procedures. Conditions have been devised under which the steroid oxidation and reduc...

synthesis of heterocyclic esters of caffeic acid using mitsunobu reaction

Large-scale Enzymatic Synthesis of 12-Ketoursodeoxycholic Acid from Dehydrocholic Acid by Simultaneous Combination of 3-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni and 7-Hydroxysteroid Dehydrogenase from Collinsella aerofaciens

12-Keto-UDCA is an important optically active component for the drug ursodeoxycholic acid (UDCA). Starting from the three-keto compound dehydrocholic acid, the carbonyl groups at position 3 and 7 have to be reduced stereoand regioselectively. In this case we applied two hydroxysteroid dehydrogenases for this purpose, the NAD-dependent 3α-HSDH from Pseudomonas testosteroni and the NADP-dependent...

Stereospecificity of hydrogen transfer by pyridine nucleotide-linked hydroxysteroid dehydrogenases.

The present studies are concerned with the determination of the steric course of the transfer of hydrogen catalyzed by hydroxysteroid dehydrogenases. The classic work of Vennesland et al. (l-3) has demonstrated that pyridine nucleotide-linked dehydrogenases catalyze a direct and stereospecific transfer of hydrogen between the substrate and the para position of the nicotinamide ring of the nucle...

synthesis of amido alkylnaphthols using nano-magnetic particles and surfactants

we used dbsa and nano-magnetic for the synthesis of amido alkylnaphtols.